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Unité de Recherche de Génomique Fonctionnelle et Physiologie des Plantes (UGFP2) de l’ISBS
 

Unité de Recherche de Génomique Fonctionnelle et Physiologie des Plantes (UGFP2) de l’ISBS.

Directeur : Pr. Moez Hanin

 

L'UGFP2 a comme double objectif d’améliorer la qualité (biofortification) et la productivité de plantes d’intérêt comme les céréales en conditions adverses (salinité, sécheresse & carence minérale) mais aussi de valoriser des protéines végétales, associées aux stress, dans des applications biotechnologiques et thérapeutiques.

L'UGFP2 est experte en génomique fonctionnelle et biologie moléculaire ainsi qu'en physiologie du stress chez les plantes. Elle possède également une bonne maitrise des techniques de génomique, transcriptomique (i.e. PCR quantitative, RNA-seq), et protéomique (i.e. protéine recombinante, co-immunoprécipitation).

Les principaux thèmes de recherche développés au sein de l'UGFP2 sont:

- Cycle cellulaire et contrôle de la biomasse végétale face aux contraintes environnementales.

- Signalisation cellulaire de la réponse des plantes aux contraintes environnementales.

- Tolérance des plantes aux carences minérales (-P) et rôle de l’acide phytique dans le contrôle de la signalisation et l'homéostasie en Pi

- Exploitation du potentiel des bactéries de type PGPR (Plant Promoting Growth Rhizobacteria) dans l'amélioration de la tolérance des plantes aux stress de l'environnement

- Valorisation biotechnologique des macromolécules végétales associées aux stress

 

 

 

UGFP2 aims at improving the quality (biofortification) and the productivity of plants of economic importance like wheat in adverse conditions (salinity, drought & mineral deficiency) but also to valorize plant stress associated proteins in diverse biotechnological applications.

UGFP2 has expertise in plant functional genomics and molecular biology as well as in stress physiology in plants. We perform genomic, transcriptomic (ie quantitative PCR, RNA-seq), and proteomic (i.e. recombinant protein, co-immunoprecipitation) techniques.

The main research topics developed within the IGFP2 are:

- Cell cycle and control of plant biomass during environmental constraints.

- Plant Cellular signaling of the response to environmental constraints.

- Plant Tolerance towards mineral deficiencies (-P) and role of phytic acid in the control of signaling and homeostasis of Pi

- Potential of Plant Promoting Growth Rhizobacteria (PGPR) bacteria in improving plant tolerance to environmental stress

- Biotechnological valorization of plant macromolecules associated to stress

 

Publications :

2018

Ghorbel M, Zaidi I Ebel C, Hanin M (2018) Differential regulation of the durum wheat MAPK Phosphatase 1 by calmodulin, bivalent cations and possibly Mitogen Activated Protein Kinase 3. Plant Physiol Biochem. 135: 242-252. doi: 10.1016/j.plaphy.2018

Mahjoubi H, Tamari Y, Takeda S, Bouchabké-Coussa O, Hanin M, Herzog E, Schmit AC, Chabouté ME, Ebel C (2018) The wheat TdRL1 is the functional homolog of the rice RSS1 and promotes plant salt stress tolerance. Plant Cell Rep. 37:1625-1637. doi: 10.1007/s00299-018-2333-2.

Ebel C, BenFeki A, Hanin M, Solano R, Chini A. (2018) Characterization of wheat (Triticum aestivum) TIFY family and role of Triticum Durum TdTIFY11a in salt stress tolerance. PLoS One. 2018 Jul 18;13(7):e0200566. doi: 10.1371/journal.pone.0200566.

Bradai M, Mahjoubi H, Chini A, Chabouté ME, Hanin M, Ebel C (2018) Genome wide identification of wheat and Brachypodium type one protein phosphatases and functional characterization of durum wheat TdPP1a. PLOS ONE. 13(1): e0191272

Belgaroui N, Lacombe B, Rouached H, Hanin M (2018). Phytase overexpression in Arabidopsis improves plant growth under osmotic stress and in combination with phosphate deficiency. Scientific Reports. 8(1):1137. doi: 10.1038/s41598-018-19493-w.

2017

Ghorbel M, Cotelle V, Ebel C, Zaidi I, Ormancey M, Galaud JP, Hanin M (2017) Regulation of the wheat MAP kinase phosphatase 1 by 14-3-3 proteins. Plant Sci. 257: 37-47.

Secco D, Bouain N, Rouached A, Prom-U-Thai C, Hanin M, Pandey AK, Rouached H (2017) Phosphate, phytate and phytases in plants: from fundamental knowledge gained in Arabidopsis to potential biotechnological applications in wheat. Crit. Rev. Biotech. 12: 1-13.

2016

Hanin M, Ebel C, Ngom M, Laplaze L, Masmoudi K (2016) New insights on plant salt tolerance mechanisms and their potential use for breeding. Front. Plant Sci. 7: 1787.

Belgaroui N, Berthomieu P, Rouached H, Hanin M (2016) The secretion of the bacterial phytase PHY-US417 by Arabidopsis roots reveals its potential for increasing phosphate acquisition and biomass production during co-growth. Plant Biotechnol. J. 14: 1914-1924.

Zaidi I, Ebel C, Belgaroui N, Ghorbel M, Amara I,  Hanin M (2016) The wheat MAP kinase phosphatase 1 alleviates salt stress and increases antioxidant activities in Arabidopsis. J. Plant Physiol. 193: 12-21.

2015

Ebel C, Hanin M (2015) Maintenance of meristem activity under stress: is there an interplay of RSS1-like proteins with the RBR pathway? Plant Biol. (Stuttg). 18:167-170.

Mahjoubi H, Ebel C, Hanin M (2015) Molecular and functional characterization of the durum wheat TdRL1, a member of the conserved Poaceae RSS1-like family that exhibits features of intrinsically disordered proteins and confers stress tolerance in yeast. Funct. Integr. Genomics. 15:717-728.


Membres de l’équipe de recherche :

 

Professeur Moez Hanin : moez.hanin@isbs.rnu.tn

Professeur Nabil Miled : nabil.miled@isbs.rnu.tn

Professeur Yassine Ben Ali : yassine.benali@isbs.usf.tn

Dr. Sofiane Ghorbel : sofiane.ghorbel@isbs.rnu.tn

Dr. Chantal Ebel : chantal.ebel@isbs.rnu.tn

Dr. Samiha Mezghani 

Dr. Baccouche Salem

Dr. Drira Sihem

Dr. Hadhri Boutheina

Dr. Elarbi Mahdi

Hamza Fetoui: étudiant en Master

Naima Sayahi : étudiante stagiaire